![]() Samples need to be at the medium to upper concentration range for a successful run (additional fees will apply if the sample is not diluted appropriately).įor any of these services, please provide at least 2 ul of sample in labelled 0.5 or 1.0 ml tubes. If you would like us to pool your libraries we can do this too (pooling is typically based on Qubit measurements, unless the libraries are PCR-free).īioanalyzer QC is offered on the DNA High Sensitivity, DNA 1000, DNA 12000 or RNA Nano chips. Quantification of double-stranded DNA using the Qubit Fluorometer (Life Technologies) is also available as a service. When analyzing a larger number number of samples or libraries (> 24) the LabChip Gx instrument offers a more affordable and faster alternative to the bioanalyzer. This kit measures absolute numbers of molecules containing the Illumina adapter sequences, thus providing a highly accurate measurement of amplifiable molecules available for cluster generation. However, if you are preparing libraries and need them quantified (such as PCR-free libraries prior to pooling), we offer a stand-alone qPCR service using the KAPA Biosystems SYBR FAST ‘Library Quantification Kit for Illumina Sequencing Platforms. Our library preparation service includes Bioanalyzer QC, and our sequencing service includes qPCR quantification. Use the methods listed in the tables below to validate the final library before sequencing.Library quality control (QC) is essential prior to sequencing. In this bulletin, we summarize the library quantification and quality control methods for libraries prepared with all current Illumina kits. There are different methods for quantification and quality control depending on the sequencing library kit being used. Accurate quantification and proper quality check of next-generation sequencing libraries is key to a successful sequencing run. ![]()
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